Application

Research CategorySignaling

Immunoblot: 1:500-1:1000; antibody recognizes all isoforms, the cPLA.

Alpha (85kDa) typically migrates as 100-110kDa on reducing westerns; beta migrates as 114kDa; antibody will also recognize the sPLA at approximately 14-20kDa.

ELISA

Optimal working dilutions must be determined by the end user.

Detect Phospholipase A2 using this Anti-Phospholipase A2 Antibody, clone CH-7 validated for use in ELISA & WB.

Research Sub CategoryLipid Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant cPLA2 expressed from the human cPLA2 gene.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

In 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6

Specificity

Antibody reacts with cytosolic form of PLA2. Antibody will also react with beta isoform. Epitope has been localized within the first 216 aa of the N-terminal Ca++ binding domain of cPLA2

Storage and Stability

Maintain at 2-8°C.